Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GABBR1 / GABA B Receptor 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110563-100
Catalog Number: LS-G110563
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GABA B Receptor 1(GABBR1) (NM_021904), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ271M21.1.1 | DJ271M21.1.2 | GABA(B) receptor | Gaba-b-r1a | GABA-BR1 | Gaba-br1a receptor | GABAB | subunit 1c | GABAB1 | GABA-B receptor 1 | Gaba-b1 | Gababr1 receptor | GPRC3A | Gabab-receptor subunit 1a | GABABR1 | GABBR1 | GABBR1-3 | GBR1 | HGB1a | GABA-B-R1 | GB1
- Origin Species
- Human
- NCBI GenBank
- NM_001470
- SwissProt
- Q9UBS5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 99.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLLLLLAPL FLRPPGAGGA QTPNATSEGC QIIHPPWEGG IRYRGLTRDQ VKAINFLPVD61YEIEYVCRGE REVVGPKVRK CLANGSWTDM DTPSRCVNRT PHSERRAVYI GALFPMSGGW121PGGQACQPAV EMALEDVNSR RDILPDYELK LIHHDSKCDP GQATKYLYEL LYNDPIKIIL181MPGCSSVSTL VAEAARMWNL IVLSYGSSSP ALSNRQRFPT FFRTHPSATL HNPTRVKLFE241KWGWKKIATI QQTTEVFTST LDDLEERVKE AGIEITFRQS FFSDPAVPVK NLKRQDARII301VGLFYETEAR KVFCEVYKER LFGKKYVWFL IGWYADNWFK IYDPSINCTV DEMTEAVEGH361ITTEIVMLNP ANTRSISNMT SQEFVEKLTK RLKRHPEETG GFQEAPLAYD AIWALALALN421KTSGGGGRSG VRLEDFNYNN QTITDQIYRA MNSSSFEGVS GHVVFDASGS RMAWTLIEQL481QGGSYKKIGY YDSTKDDLSW SKTDKWIGGS PPADQTLVIK TFRFLSQKLF ISVSVLSSLG541IVLAVVCLSF NIYNSHVRYI QNSQPNLNNL TAVGCSLALA AVFPLGLDGY HIGRNQFPFV601CQARLWLLGL GFSLGYGSMF TKIWWVHTVF TKKEEKKEWR KTLEPWKLYA TVGLLVGMDV661LTLAIWQIVD PLHRTIETFA KEEPKEDIDV SILPQLEHCS SRKMNTWLGI FYGYKGLLLL721LGIFLAYETK SVSTEKINDH RAVGMAIYNV AVLCLITAPV TMILSSQQDA AFAFASLAIV781FSSYITLVVL FVPKMRRLIT RGEWQSEAQD TMKTGSSTNN NEEEKSRLLE KENRELEKII841AEKEERVSEL RHQLQSRQQL RSRRHPPTPP EPSGGLPRGP PEPPDRLSCD GSRVHLLYKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
899 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.