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GAB4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GAB4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120547-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GAB4 (NM_001037814), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GAB2-like | GAB4 | GRB2-associated binder 2-like | GRB2-associated binder 4
Origin Species
Human
NCBI GenBank
NM_001037814
SwissProt
Q2WGN9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLPSPSPSR ELCPPDPAFA PLSSWPGSGP AGGSTRSGHV LYSGWLRKSP PEKKLRLFAW
61RKRWFILRRG QTSSDPDVLE YYKNDGSKKP LRTINLNLCE QLDVDVTLNF NKKEIQKGYM
121FDIKTSERTF YLVAETREDM NEWVQSICQI CGFRQEESTG FLGNISSASH GLCSSPAEPS
181CSHQHLPQEQ EPTSEPPVSH CVPPTWPIPA PPGCLRSHQH ASQRAEHARS ASFSQGSEAP
241FIMRRNTAMQ NLAQHSGYSV DGVSGHIHGF HSLSKPSQHN AEFRGSTHRI PWSLASHGHT
301RGSLTGSEAD NEASSGKYTQ HGGGNASRPA ESMHEGVCSF LPGRTLVGLS DSIASEGSCV
361PMNPGSPTLP AVKQAGDDSQ GVCIPVGSCL VRFDLLGSPL TELSMHQDLS QGHEVQLPPV
421NRSLKPNQKA NPTPPNLRNN RVINELSFKP PVTEPWSGTS HTFDSSSSQH PISTQSITNT
481DSEDSGERYL FPNPASAFPV SGGTSSSAPP RSTGNIHYAA LDFQPSKPSI GSVTSGKKVD
541YVQVDLEKTQ ALQKTMHEQM CLRQSSEPPR GAKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

574 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.