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GAB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GAB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107860-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GAB3 (NM_080612), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DOS/Gab family member 3 | Gab3 scaffolding protein | GAB3 | GRB2-associated binder 3
Origin Species
Human
SwissProt
Q8WWW8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAGDAVCTG WLVKSPPERK LQRYAWRKRW FVLRRGRMSG NPDVLEYYRN KHSSKPIRVI
61DLSECAVWKH VGPSFVRKEF QNNFVFIVKT TSRTFYLVAK TEQEMQVWVH SISQVCNLGH
121LEDGADSMES LSYTPSSLQP SSASSLLTAH AASSSLPRDD PNTNAVATEE TRSESELLFL
181PDYLVLSNCE TGRLHHTSLP TRCDSWSNSD RSLEQASFDD VFVDCLQPLP SSHLVHPSCH
241GSGAQEVPSS RPQAALIWSR EINGPPRDHL SSSPLLESSL SSTIQVDKNQ GSLPCGAKEL
301DIMSNTPPPR PPKPSHLSER RQEEWSTHSG SKKPECTLVP RRISLSGLDN MRTWKADVEG
361QSLRHRDKRL SLNLPCRFSP MYPTASASIE DSYVPMSPQA GASGLGPHCS PDDYIPMNSG
421SISSPLPELP ANLEPPPVNR DLKPQRKSRP PPLDLRNLSI IREHASLTRT RTVPCSRTSF
481LSPERNGINS ARFFANPVSR EDEESYIEME EHRTASSLSS GALTWTKKFS LDYLALDFNS
541ASPAPMQQKL LLSEEQRVDY VQVDEQKTQA LQSTKQEWTD ERQSKV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

586 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.