Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FZD9 / Frizzled 9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117288-100
Catalog Number: LS-G117288
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Frizzled 9(FZD9) (NM_003508), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD349 | Frizzled homolog 9 | Fz-9 | Frizzled family receptor 9 | Frizzled-9 | Frizzled 9 | Fz9 | HFz9 | CD349 antigen | Frizzled homolog fzd3 | FZD9 | FzE6
- Origin Species
- Human
- NCBI GenBank
- NM_003508
- SwissProt
- O00144
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAVAPLRGAL LLWQLLAAGG AALEIGRFDP ERGRGAAPCQ AVEIPMCRGI GYNLTRMPNL61LGHTSQGEAA AELAEFAPLV QYGCHSHLRF FLCSLYAPMC TDQVSTPIPA CRPMCEQARL121RCAPIMEQFN FGWPDSLDCA RLPTRNDPHA LCMEAPENAT AGPAEPHKGL GMLPVAPRPA181RPPGDLGPGA GGSGTCENRE KFQYVEKSRS CAPRCGPGVE VFWSRRDKDF ALVWMAVWSA241LCFFSTAFTV LTFLLEPHRF QYPERPIIFL SMCYNVYSLA FLIRAVAGAQ SVACDQEAGA301LYVIQEGLEN TGCTLVFLLL YYFGMASSLW WVVLTLTWFL AAGKKWGHEA IEAHGSYFHM361AAWGLPALKT IVILTLRKVA GDELTGLCYV ASTDAAALTG FVLVPLSGYL VLGSSFLLTG421FVALFHIRKI MKTGGTNTEK LEKLMVKVGV FSILYTVPAT CVIVCYVYER LNMDFWRLRA481TEQPCAAAAG PGGRRDCSLP GGSVPTVAVF MLKIFMSLVV GITNGVWVWS SKTFQTWQSL541CYRKIAAGRA RAKACHAPGS YGRGTHCHYK APTVVLHMTK TDPSLENPTH LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
591 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.