LSBio
FYCO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FYCO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FYCO1 antibodies →
$725.00each
Catalog Number: LS-G109947-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FYCO1 (NM_024513), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CATC2 | ZFYVE7 | FYCO1
Origin Species
Human
NCBI GenBank
NM_024513
SwissProt
Q9BQS8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
166.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASTNAESQL QRIIRDLQDA VTELSKEFQE AGEPITDDST SLHKFSYKLE YLLQFDQKEK
61ATLLGNKKDY WDYFCACLAK VKGANDGIRF VKSISELRTS LGKGRAFIRY SLVHQRLADT
121LQQCFMNTKV TSDWYYARSP FLQPKLSSDI VGQLYELTEV QFDLASRGFD LDAAWPTFAR
181RTLTTGSSAY LWKPPSRSSS MSSLVSSYLQ TQEMVSNFDL NSPLNNEALE GFDEMRLELD
241QLEVREKQLR ERMQQLDREN QELRAAVSQQ GEQLQTERER GRTAAEDNVR LTCLVAELQK
301QWEVTQATQN TVKELQTCLQ GLELGAAEKE EDYHTALRRL ESMLQPLAQE LEATRDSLDK
361KNQHLASFPG WLAMAQQKAD TASDTKGRQE PIPSDAAQEM QELGEKLQAL ERERTKVEEV
421NRQQSAQLEQ LVKELQLKED ARASLERLVK EMAPLQEELS GKGQEADQLW RRLQELLAHT
481SSWEEELAEL RREKKQQQEE KELLEQEVRS LTRQLQFLET QLAQVSQHVS DLEEQKKQLI
541QDKDHLSQQV GMLERLAGPP GPELPVAGEK NEALVPVNSS LQEAWGKPEE EQRGLQEAQL
601DDTKVQEGSQ EEELRQANRE LEKELQNVVG RNQLLEGKLQ ALQADYQALQ QRESAIQGSL
661ASLEAEQASI RHLGDQMEAS LLAVRKAKEA MKAQMAEKEA ILQSKEGECQ QLREEVEQCQ
721QLAEARHREL RALESQCQQQ TQLIEVLTAE KGQQGVGPPT DNEARELAAQ LALSQAQLEV
781HQGEVQRLQA QVVDLQAKMR AALDDQDKVQ SQLSMAEAVL REHKTLVQQL KEQNEALNRA
841HVQELLQCSE REGALQEERA DEAQQREEEL RALQEELSQA KCSSEEAQLE HAELQEQLHR
901ANTDTAELGI QVCALTVEKE RVEEALACAV QELQDAKEAA SREREGLERQ VAGLQQEKES
961LQEKLKAAKA AAGSLPGLQA QLAQAEQRAQ SLQEAAHQEL NTLKFQLSAE IMDYQSRLKN
1021AGEECKSLRG QLEEQGRQLQ AAEEAVEKLK ATQADMGEKL SCTSNHLAEC QAAMLRKDKE
1081GAALREDLER TQKELEKATT KIQEYYNKLC QEVTNRERND QKMLADLDDL NRTKKYLEER
1141LIELLRDKDA LWQKSDALEF QQKLSAEERW LGDTEANHCL DCKREFSWMV RRHHCRICGR
1201IFCYYCCNNY VLSKHGGKKE RCCRACFQKL SEGPGSPDSS GSGTSQGEPS PALSPASPGP
1261QATGGQGANT DYRPPDDAVF DIITDEELCQ IQESGSSLPE TPTETDSLDP NAAEQDTTST
1321SLTPEDTEDM PVGQDSEICL LKSGELMIKV PLTVDEIASF GEGSRELFVR SSTYSLIPIT
1381VAEAGLTISW VFSSDPKSIS FSVVFQEAED TPLDQCKVLI PTTRCNSHKE NIQGQLKVRT
1441PGIYMLIFDN TFSRFVSKKV FYHLTVDRPV IYDGSDFL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1478 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.