Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FUZ / FY Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109573-100
Catalog Number: LS-G109573
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FUZ (NM_025129), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FUZ | FY | Fuzzy homolog (Drosophila) | NTD | Protein fuzzy homolog
- Origin Species
- Human
- NCBI GenBank
- NM_025129
- SwissProt
- Q9BT04
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGEEGTGGTV HLLCLAASSG VPLFCRSSRG GAPARQQLPF SVIGSLNGVH MFGQNLEVQL61SSARTENTTV VWKSFHDSIT LIVLSSEVGI SELRLERLLQ MVFGAMVLLV GLEELTNIRN121VERLKKDLRA SYCLIDSFLG DSELIGDLTQ CVDCVIPPEG SLLQEALSGF AEAAGTTFVS181LVVSGRVVAA TEGWWRLGTP EAVLLPWLVG SLPPQTARDY PVYLPHGSPT VPHRLLTLTL241LPSLELCLLC GPSPPLSQLY PQLLERWWQP LLDPLRACLP LGPRALPSGF PLHTDILGLL301LLHLELKRCL FTVEPLGDKE PSPEQRRRLL RNFYTLVTST HFPPEPGPPE KTEDEVYQAQ361LPRACYLVLG TEEPGTGVRL VALQLGLRRL LLLLSPQSPT HGLRSLATHT LHALTPLLTR421TRPLEQKLIS EEDLAANDIL DYKDDDDKV
449 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.