Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FUT4 / CD15 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118228-100
Catalog Number: LS-G118228
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FUT4 (NM_002033), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha-(1 | 3)-fucosyltransferase | CD15 | ELAM ligand fucosyltransferase | ELFT | Fucosyltransferase 4 | Fucosyltransferase IV | FucT-IV | FUT4 | LeX | FCT3A | FUC-TIV | FUTIV | Lewis X | SSEA-1
- Origin Species
- Human
- NCBI GenBank
- NM_002033
- SwissProt
- P22083
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRRLWGAARK PSGAGWEKEW AEAPQEAPGA WSGRLGPGRS GRKGRAVPGW ASWPAHLALA61ARPARHLGGA GQGPRPLHSG TAPFHSRASG ERQRRLEPQL QHESRCRSST PADAWRAEAA121LPVRAMGAPW GSPTAAAGGR RGWRRGRGLP WTVCVLAAAG LTCTALITYA CWGQLPPLPW181ASPTPSRPVG VLLWWEPFGG RDSAPRPPPD CRLRFNISGC RLLTDRASYG EAQAVLFHHR241DLVKGPPDWP PPWGIQAHTA EEVDLRVLDY EEAAAAAEAL ATSSPRPPGQ RWVWMNFESP301SHSPGLRSLA SNLFNWTLSY RADSDVFVPY GYLYPRSHPG DPPSGLAPPL SRKQGLVAWV361VSHWDERQAR VRYYHQLSQH VTVDVFGRGG PGQPVPEIGL LHTVARYKFY LAFENSQHLD421YITEKLWRNA LLAGAVPVVL GPDRANYERF VPRGAFIHVD DFPSASSLAS YLLFLDRNPA481VYRRYFHWRR SYAVHITSFW DEPWCRVCQA VQRAGDRPKS IRNLASWFERSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
530 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.