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FUBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FUBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FUBP3 antibodies →
$725.00each
Catalog Number: LS-G117001-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FUBP3 (NM_003934), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FBP3 | FUSE-binding protein 3 | FUBP3
Origin Species
Human
NCBI GenBank
U69127
SwissProt
Q96I24

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAELVQGQSA PVGMKAEGFV DALHRVRQIA AKIDSIPHLN NSTPLVDPSV YGYGVQKRPL
61DDGVGNQLGA LVHQRTVITE EFKVPDKMVG FIIGRGGEQI SRIQAESGCK IQIASESSGI
121PERPCVLTGT PESIEQAKRL LGQIVDRCRN GPGFHNDIDS NSTIQEILIP ASKVGLVIGR
181GGETIKQLQE RTGVKMVMIQ DGPLPTGADK PLRITGDAFK VQQAREMVLE IIREKDQADF
241RGVRGDFNSR MGGGSIEVSV PRFAVGIVIG RNGEMIKKIQ NDAGVRIQFK PDDGISPERA
301AQVMGPPDRC QHAAHIISEL ILTAQERDGF GGLAAARGRG RGRGDWSVGA PGGVQEITYT
361VPADKCGLVI GKGGENIKSI NQQSGAHVEL QRNPPPNSDP NLRRFTIRGV PQQIEVARQL
421IDEKVGGTNL GAPGAFGQSP FSQPPAPPHQ NTFPPRSSGC FPNMAAKVNG NPHSTPVSGP
481PAFLTQGWGS TYQAWQQPTQ QVPSQQSQPQ SSQPNYSKAW EDYYKKQSHA ASAAPQASSP
541PDYTMAWAEY YRQQVAFYGQ TLGQAQAHSQ EQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

572 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.