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FSTL5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FSTL5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111339-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FSTL5 (NM_020116), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Follistatin-like protein 5 | KIAA1263 | Follistatin-like 5 | Follistatin-related protein 5 | FSTL5
Origin Species
Human
NCBI GenBank
AB033089
SwissProt
Q8N475

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MFKCWSVVLV LGFIFLESEG RPTKEGGYGL KSYQPLMRLR HKQEKNQESS RVKGFMIQDG
61PFGSCENKYC GLGRHCVTSR ETGQAECACM DLCKRHYKPV CGSDGEFYEN HCEVHRAACL
121KKQKITIVHN EDCFFKGDKC KTTEYSKMKN MLLDLQNQKY IMQENENPNG DDISRKKLLV
181DQMFKYFDAD SNGLVDINEL TQVIKQEELG KDLFDCTLYV LLKYDDFNAD KHLALEEFYR
241AFQVIQLSLP EDQKLSITAA TVGQSAVLSC AIQGTLRPPI IWKRNNIILN NLDLEDINDF
301GDDGSLYITK VTTTHVGNYT CYADGYEQVY QTHIFQVNVP PVIRVYPESQ AREPGVTASL
361RCHAEGIPKP QLGWLKNGID ITPKLSKQLT LQANGSEVHI SNVRYEDTGA YTCIAKNEAG
421VDEDISSLFV EDSARKTLAN ILWREEGLGI GNMFYVFYED GIKVIQPIEC EFQRHIKPSE
481KLLGFQDEVC PKAEGDEVQR CVWASAVNVK DKFIYVAQPT LDRVLIVDVQ SQKVVQAVST
541DPVPVKLHYD KSHDQVWVLS WGTLEKSSPT LQVITLASGN VPHHTIHTQP VGKQFDRVDD
601FFIPTTTLII THMRFGFILH KDEAALQKID IETMSYIKTI NLKDYKCVPQ SLAYTHLGGY
661YFIGCKPDST GAVSPQVMVD GVTDSVIGFN SDVTGTPYVS PDGHYLVSIN DVKGLVRVQY
721ITIRGEIQEA FDIYTNLHIS DLAFQPSFTE AHQYNIYGSS STQTDVLFVE LSSGKVKMIK
781SLKEPLKAEE WPWNRKNRQI QDSGLFGQYL MTPSKDSLFI LDGRLNKLNC EITEVEKGNT
841VIWVGDA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

847 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.