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FSIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FSIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106129-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FSIP1 (NM_152597), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FSIP1 | HSD10
Origin Species
Human
SwissProt
Q8NA03

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDIIKGNLDG ISKPASNSRI RPGSRSSNAS LEVLSTEPGS FKVDTASNLN SGKEDHSESS
61NTENRRTSND DKQESCSEKI KLAEEGSDED LDLVQHQIIS ECSDEPKLKE LDSQLQDAIQ
121KMKKLDKILA KKQRREKEIK KQGLEMRIKL WEEIKSAKYS EAWQSKEEME NTKKFLSLTA
181VSEETVGPSH EEEDTFSSVF HTQIPPEEYE MQMQKLNKDF TCDVERNESL IKSGKKPFSN
241TEKIELRGKH NQDFIKRNIE LAKESRNPVV MVDREKKRLV ELLKDLDEKD SGLSSSEGDQ
301SGWVVPVKGY ELAVTQHQQL AEIDIKLQEL SAASPTISSF SPRLENRNNQ KPDHDGERNM
361EVTPGEKILR NTKEQRDLHN RLREIDEKLK MMKENVLEST SRLSEEQLKC LLDECILKQK
421SIIKLSSERK KEDIEDVTPV FPQLSRSIIS KLLNESETKV QKTEVEDADM LESEECEASK
481GYYLTKALTG HNMSEALVTE AENMKCLQFS KDVIISDTKD YFMSKTLGIG RLKRPSFLDD
541PLYGISVSLS SEDQHLKLSS PENTIADEQE TKDAAEECKE P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

581 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.