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FSD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FSD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FSD2 antibodies →
$725.00each
Catalog Number: LS-G121336-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FSD2 (NM_001007122), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FSD2 | SPRY domain containing 1 | RP11-127F21 | SPRYD1
Origin Species
Human
SwissProt
A1L4K1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEESGEELG LDRSTPKDFH FYHMDLYDSE DRLHLFPEEN TRMRKVVQAE MANESRGAGD
61GKAQRDLQEE VDELVHLYGL EDDHELGDEF VDENIPRTGV SEYPPYMMKR RDPAREQRDW
121RLSGEAAEAE DLGFGGWGSA GQCQDLREAY RYTHGRASEE YECYVIPEEE DEEEAADVFC
181VTCKTPIRAF QKVFDEHKEH EVIPLNEALE SAKDEIHKNM YKLEKQIIEM ENFANHLEEV
241FITVEENFGK QEQNFESHYN EILETLAQKY EEKIQALGEK KKEKLEALYG QLVSCGENLD
301TCKELMETIE EMCHEEKVDF IKDAVAMADR LGKFLKTKTD VEISAQPEFE DQTLDFSDVE
361QLMGSINTIP APSAPVINPQ VPNSATGSSV RVCWSLYSDD TVESYQLSYR PVQDSSPGTD
421QAEFTVTVKE TYCSVTNLVP NTQYEFWVTA HNRAGPSPSS ERAVYMTAPS PPIIKTKEIR
481SCEEAVLICW ESGNLNPVDS YTVELTQAES PEASGVTESV VGIPTCESVV QLQPGRSYII
541YVRALNMGGP SVRSEPATVH TIGSYFRLNK DTCHPWLTIS EDGLTAVRSE RRTPARELSP
601SDTHFTRCVA VMGNLIPVRG HHYWEVEVDE HLDYRVGVAF ADVRKQEDLG ANCLSWCMRH
661TFASSRHKYE FLHNRTTPDI RITVPPKKIG ILLDYEHSKL SFFNVDLSQH LYTFSCQLHE
721FVHPCFSLEK PGCLKVHNGI SMPKHVTFY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

749 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.