LSBio
FSCN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FSCN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FSCN3 antibodies →
$504.00each
Catalog Number: LS-G111199-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Fascin 3(FSCN3) (NM_020369), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Fascin-3 | Testis fascin | FSCN3
Origin Species
Human
NCBI GenBank
NM_020369
SwissProt
Q9NQT6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MDETEWIHRH PKAEDLRVGL ISWAGTYLTF EACKNTVTAT AKSLGRRQTW EILVSNEHET
61QAVVRLKSVQ GLYLLCECDG TVCYGRPRTS HHGCFLLRFH RNSKWTLQCL ISGRYLESNG
121KDVFCTSHVL SAYHMWTPRP ALHVHVILYS PIHRCYARAD PTMGRIWVDA AVPCLEECGF
181LLHFRDGCYH LETSTHHFLS HVDRLFSQPS SQTAFHMQVR PGGLVALCDG EGGMLYPQGT
241HLLLGMGCNP MRGEEWFILQ HCPTWVSLRS KTGRFISVIY DGEVRAASER LNRMSLFQFE
301CDSESPTVQL RSANGYYLSQ RRHRAVMADG HPLESDTFFR MHWNCGRIIL QSCRGRFLGI
361APNSLLMANV ILPGPNEEFG ILFANRSFLV LRGRYGYVGS SSGHDLIQCN QDQPDRIHLL
421PCRPGIYHFQ AQGGSFWSIT SFGTFRPWGK FALNFCIELQ GSNLLTVLAP NGFYMRADQS
481GTLLADSEDI TRECIWEF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

498 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.