Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FSAPc / C19orf29 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119696-100
Catalog Number: LS-G119696
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CACTIN (NM_001080543), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cactin homolog | C19orf29 | FSAPc | NY-REN-24 | NY REN 24 antigen | NY-REN-24 antigen | CACTIN
- Origin Species
- Human
- SwissProt
- Q8WUQ7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRDTRSRSR SAGRRGRRRQ SQSGSRSRSR SHGRRNRRRR EDEGRRRRRR RSRERRSDSE61EERWQRSGMR SRSPPRPKWH SRDGSSQSDS GEEQSRGQWA RRRRRARSWS PSSSASSSAS121PGRSQSPRAA AAALSQQQSL QERLRLREER KQQEELMKAF ETPEEKRARR LAKKEAKERK181KREKMGWGEE YMGYTNTDNP FGDNNLLGTF IWNKALEKKG ISHLEEKELK ERNKRIQEDN241RLELQKVKQL RLEREREKAM REQELEMLQR EKEAEHFKTW EEQEDNFHLQ QAKLRSKIRI301RDGRAKPIDL LAKYISAEDD DLAVEMHEPY TFLNGLTVAD MEDLLEDIQV YMELEQGKNA361DFWRDMTTIT EDEISKLRKL EASGKGPGER REGVNASVSS DVQSVFKGKT YNQLQVIFQG421IEGKIRAGGP NLDMGYWESL LQQLRAHMAR ARLRERHQDV LRQKLYKLKQ EQGVESEPLF481PILKQEPQSP SRSLEPEDVA PTPPGPSSEG GPAEAEVDGA TPTEGDGDGD GEGEGEGEAV541LMEEDLIQQS LDDYDAGRYS PRLLTAHELP LDAHVLEPDE DLQRLQLSRQ QLQVTGDASE601SAEDIFFRRA KEGMGQDEAQ FSVEMPLTGK AYLWADKYRP RKPRFFNRVH TGFEWNKYNQ661THYDFDNPPP KIVQGYKFNI FYPDLIDKRS TPEYFLEACA DNKDFAILRF HAGPPYEDIA721FKIVNREWEY SHRHGFRCQF ANGIFQLWFH FKRYRYRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
758 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.