Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FSAP57 / SMU1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111882-100
Catalog Number: LS-G111882
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMU1 (NM_018225), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BWD | FSAP57 | SMU1 | RP11-54K16.3 | SMU-1
- Origin Species
- Human
- NCBI GenBank
- NM_018225
- SwissProt
- Q2TAY7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSIEIESSDV IRLIMQYLKE NSLHRALATL QEETTVSLNT VDSIESFVAD INSGHWDTVL61QVIQSLKLPD KTLIDLYEQV VLELIELREL GAARSLLRQT DPMIMLKQTQ PERYIHLENL121LARSYFDPRE AYPDGSSKEK RRAAIAQALA GEVSVVPPSR LMALLGQALK WQQHQGLLPP181GMTIDLFRGK AAVKDVEEEK FPTQLSRHIK FGQKSHVECA RFSPDGQYLV TGSVDGFIEV241WNFTTGKIRK DLKYQAQDNF MMMDDAVLCM CFSRDTEMLA TGAQDGKIKV WKIQSGQCLR301RFERAHSKGV TCLSFSKDSS QILSASFDQT IRIHGLKSGK TLKEFRGHSS FVNEATFTQD361GHYIISASSD GTVKIWNMKT TECSNTFKSL GSTAGTDITV NSVILLPKNP EHFVVCNRSN421TVVIMNMQGQ IVRSFSSGKR EGGDFVCCAL SPRGEWIYCV GEDFVLYCFS TVTGKLERTL481TVHEKDVIGI AHHPHQNLIA TYSEDALLKL WKPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
513 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.