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FRMD6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FRMD6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FRMD6 antibodies →
$504.00each
Catalog Number: LS-G119544-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FRMD6 (NM_001042481), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf31 | Expanded homolog | FERM domain containing 6 | EX1 | FRMD6 | Willin | C14_5320
Origin Species
Human
NCBI GenBank
NM_001042481
SwissProt
Q8VII0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNKLNFHNNR VMQDRRSVCI FLPNDESLNI IINVKILCHQ LLVQVCDLLR LKDCHLFGLS
61VIQNNEHVYM ELSQKLYKYC PKEWKKEASK GIDQFGPPMI IHFRVQYYVE NGRLISDRAA
121RYYYYWHLRK QVLHSQCVLR EEAYFLLAAF ALQADLGNFK RNKHYGKYFE PEAYFPSWVV
181SKRGKDYILK HIPNMHKDQF ALTASEAHLK YIKEAVRLDD VAVHYYRLYK DKREIEASLT
241LGLTMRGIQI FQNLDEEKQL LYDFPWTNVG KLVFVGKKFE ILPDGLPSAR KLIYYTGCPM
301RSRHLLQLLS NSHRLYMNLQ PVLRHIRKLE ENEEKKQYRE SYISDNLDLD MDQLEKRSRA
361SGSSAGSMKH KRLSRHSTAS HSSSHTSGIE ADTKPRDTGP EDSYSSSAIH RKLKTCSSMT
421SHGSSHTSGV ESGGKDRLEE DLQDDEIEML VDDPRDLEQM NEESLEVSPD MCIYITEDML
481MSRKLNGHSG LIVKEIGSST SSSSETVVKL RGQSTDSLPQ TICRKPKTST DRHSLSLDDI
541RLYQKDFLRI AGLCQDTAQS YTFGCGHELD EEGLYCNSCL AQQCINIQDA FPVKRTSKYF
601SLDLTHDEVP EFVV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

614 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.