LSBio
FRMD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FRMD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FRMD1 antibodies →
$725.00each
Catalog Number: LS-G109706-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FRMD1 (NM_024919), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FERM domain containing 1 | BA164L23.1 | FRMD1
Origin Species
Human
NCBI GenBank
NM_024919
SwissProt
Q8N878

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVPPRGRGI DPARTNPDTF PPSGARCMEP SPERPACSQQ EPTLGMDAMA SEHRDVLVLL
61PSREQLRLAV GVKATGRELF QQVCNVASIR DAQFFGLCVV RNNEYIFMDL EQKLSKYFSK
121DWKKERNEGN EKPRAPFVAF LRVQHYVENG RVISDHRARH LYYCHLKERV LRSQCAHREE
181AYFLLAACAL QADLGEHRES AHAGRYFEPH SYFPQWIITK RGIDYILRHM PTLHRERQGL
241SPKEAMLCFI QEACRLEDVP VHFFRLHKDK KEGRPTVILG LALRGVHIYQ GKKLEIQLDG
301LPAAQKLVYY TGCTWRSRHL LHLLRASHQL HLRVRPTLQQ LRQREEAEEK QHYRESYISD
361ELELDLASRS FPGSGVSSQH CPHCLSRHSA DSHGSSYTSG IKANSWLRES REMSVDVPLE
421VHGLHEKEPS SSPRTSRSHP STRGDSQATR QEPCTEVRTR GQSAEAVHQI QEMTAGVSEE
481QHSHGLDDMQ LHQLALHPAP TSLSHTFHRA LDCRLAGPCE TRATLPSKRS SNCLALDLFG
541EAPPQEFVV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

549 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.