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FPGT Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FPGT Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117055-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FPGT (NM_003838), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FPGT | GDP-L-fucose diphosphorylase | GDP-L-fucose pyrophosphorylase | GFPP
Origin Species
Human
NCBI GenBank
NM_003838
SwissProt
O14772

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAARDPPEV SLREATQRKL RRFSELRGKL VARGEFWDIV AITAADEKQE LAYNQQLSEK
61LKRKELPLGV QYHVFVDPAG AKIGNGGSTL CALQCLEKLY GDKWNSFTIL LIHSGGYSQR
121LPNASALGKI FTALPLGNPI YQMLELKLAM YIDFPLNMNP GILVTCADDI ELYSIGEFEF
181IRFDKPGFTA LAHPSSLTIG TTHGVFVLDP FDDLKHRDLE YRSCHRFLHK PSIEKMYQFN
241AVCRPGNFCQ QDFAGGDIAD LKLDSDYVYT DSLFYMDHKS AKMLLAFYEK IGTLSCEIDA
301YGDFLQALGP GATVEYTRNT SNVIKEESEL VEMRQRIFHL LKGTSLNVVV LNNSKFYHIG
361TTEEYLFYFT SDNSLKSELG LQSITFSIFP DIPECSGKTS CIIQSILDSR CSVAPGSVVE
421YSRLGPDVSV GENCIISGSY ILTKAALPAH SFVCSLSLKM NRCLKYATMA FGVQDNLKKS
481VKTLSDIKLL QFFGVCFLSC LDVWNLKVTE ELFSGNKTCL SLWTARIFPV CSSLSDSVIT
541SLKMLNAVKN KSAFSLNSYK LLSIEEMLIY KDVEDMITYR EQIFLEISLK SSLM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

594 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.