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FOXRED2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FOXRED2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FOXRED2 antibodies →
$725.00each
Catalog Number: LS-G118788-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FOXRED2 (NM_001102371), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ERFAD | FOXRED2
Origin Species
Human
NCBI GenBank
NM_024955
SwissProt
Q8IWF2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MGLSAAAPLW GPPGLLLAIA LHPALSVPPR RDYCVLGAGP AGLQMAYFLQ RAGRDYAVFE
61RAPRPGSFFT RYPRHRKLIS INKRYTGKAN AEFNLRHDWN SLLSHDPRLL FRHYSRAYFP
121DARDMVRYLG DFADTLGLRV QYNTTIAHVT LDKDRQAWNG HYFILTDQKG QVHQCSVLFV
181ATGLSVPNQV DFPGSEYAEG YESVSVDPED FVGQNVLILG RGNSAFETAE NILGVTNFIH
241MLSRSRVRLS WATHYVGDLR AINNGLLDTY QLKSLDGLLE SDLTDLAILK DSKGKFHVTP
301KFFLEEANTN QSADSITLPQ DDNDNFAMRV PYDRVIRCLG WNFDFSIFNK SLRLNSGNAF
361GKKYPLIRAS YESKGSRGLF ILGTASHSVD YRKSAGGFIH GFRYTVRAVH RLLEHRHHSV
421TWPATELPIT QLTSSIVRRV NEASGLYQMF GVLADVILLK ENSTAFEYLE EFPIQMLAQL
481ETLTGRKAKH GLFVINMEYG RNFSGPDKDV FFDDRSVGHT EDAWQSNFLH PVIYYYRYLP
541TEQEVRFRPA HWPLPRPTAI HHIVEDFLTD WTAPIGHILP LRRFLENCLD TDLRSFYAES
601CFLFALTRQK LPPFCQQGYL RMQGLVSTES LWQHRVESRL LRDYAPTGRR LEDSSQQLGD
661QEPLGSPLAP GPLAQSVDSN KEEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

684 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.