LSBio
FOXP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FOXP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106477-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FOXP2 (NM_148899), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CAG repeat protein 44 | FOXP2 | Forkhead box protein P2 | Forkhead box P2 | SPCH1 | CAGH44 | TNRC10
Origin Species
Human
NCBI GenBank
NM_014491
SwissProt
O15409

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMTPQVITPQ QMQQILQQQV LSPQQLQALL QQQQAVMLQQ QQLQEFYKKQ QEQLHLQLLQ
61QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQH PGKQAKEQQQ QQQQQQQLAA
121QQLVFQQQLL QMQQLQQQQH LLSLQRQGLI SIPPGQAALP VQSLPQAGLS PAEIQQLWKE
181VTGVHSMEDN GIKHGGLDLT TNNSSSTTSS NTSKASPPIT HHSIVNGQSS VLSARRDSSS
241HEETGASHTL YGHGVCKWPG CESICEDFGQ FLKHLNNEHA LDDRSTAQCR VQMQVVQQLE
301IQLSKERERL QAMMTHLHMR PSEPKPSPKP LNLVSSVTMS KNMLETSPQS LPQTPTTPTA
361PVTPITQGPS VITPASVPNV GAIRRRHSDK YNIPMSSEIA PNYEFYKNAD VRPPFTYATL
421IRQAIMESSD RQLTLNEIYS WFTRTFAYFR RNAATWKNAV RHNLSLHKCF VRVENVKGAV
481WTVDEVEYQK RRSQKITGSP TLVKNIPTSL GYGAALNASL QAALAESSLP LLSNPGLINN
541ASSGLLQAVH EDLNGSLDHI DSNGNSSPGC SPQPHIHSIH VKEEPVIAED EDCPMSLVTT
601ANHSPELEDD REIEEEPLSE DLE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

623 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.