Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FOXK2 / ILF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116598-100
Catalog Number: LS-G116598
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ILF1(FOXK2) (NM_004514), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ILF1 | ILF-1 | Forkhead box K2 | ILF | Forkhead box protein K2 | FOXK2
- Origin Species
- Human
- NCBI GenBank
- NM_004514
- SwissProt
- Q01167
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAAAALSG AGTPPAGGGA GGGGAGGGGS PPGGWAVARL EGREFEYLMK KRSVTIGRNS61SQGSVDVSMG HSSFISRRHL EIFTPPGGGG HGGAAPELPP AQPRPDAGGD FYLRCLGKNG121VFVDGVFQRR GAPPLQLPRV CTFRFPSTNI KITFTALSSE KREKQEASES PVKAVQPHIS181PLTINIPDTM AHLISPLPSP TGTISAANSC PSSPRGAGSS GYKVGRVMPS DLNLMADNSQ241PENEKEASGG DSPKDDSKPP YSYAQLIVQA ITMAPDKQLT LNGIYTHITK NYPYYRTADK301GWQNSIRHNL SLNRYFIKVP RSQEEPGKGS FWRIDPASES KLIEQAFRKR RPRGVPCFRT361PLGPLSSRSA PASPNHAGVL SAHSSGAQTP ESLSREGSPA PLEPEPGAAQ PKLAVIQEAR421FAQSAPGSPL SSQPVLITVQ RQLPQAIKPV TYTVATPVTT STSQPPVVQT VHVVHQIPAV481SVTSVAGLAP ANTYTVSGQA VVTPAAVLAP PKAEAQENGD HREVKVKVEP IPAIGHATLG541TASRIIQTAQ TTPVQTVTIV QQAPLGQHQL PIKTVTQNGT HVASVPTAVH GQVNNAAASP601LHMLATHASA SASLPTKRHN GDQPEQPELK RIKTEDGEGI VIALSVDTPP AAVREKGVQNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
660 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.