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FOXJ2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FOXJ2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G101565-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FOXJ2 (NM_018416), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Fork head homologous X | Forkhead box J2 | FOXJ2 | FOXJ2 forkhead factor | FHX | Forkhead box protein J2
Origin Species
Human
NCBI GenBank
NM_018416
SwissProt
Q9P0K8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASDLESSLT SIDWLPQLTL RATIEKLGSA SQAGPPGSSR KCSPGSPTDP NATLSKDEAA
61VHQDGKPRYS YATLITYAIN SSPAKKMTLS EIYRWICDNF PYYKNAGIGW KNSIRHNLSL
121NKCFRKVPRP RDDPGKGSYW TIDTCPDISR KRRHPPDDDL SQDSPEQEAS KSPRGGVAGS
181GEASLPPEGN PQMSLQSPTS IASYSQGTGS VDGGAVAAGA SGRESAEGPP PLYNTNHDFK
241FSYSEINFQD LSWSFRNLYK SMLEKSSSSS QHGFSSLLGD IPPSNNYYMY QQQQPPPPQQ
301QQQQQQPPQP PPQQSQPQQQ QAPAQGPSAV GGAPPLHTPS TDGCTPPGGK QAGAEGYGPP
361PVMAMHPPPL QHGGYHPHQH HPHSHPAQQP PPPQPQAQGQ APINNTGFAF PSDWCSNIDS
421LKESFKMVNR LNWSSIEQSQ FSELMESLRQ AEQKNWTLDQ HHIANLCDSL NHFLTQTGHV
481PPQGGTHRPP APARIADSCA LTSGKQESAM SQVNSYGHPQ APHLYPGPSP MYPIPTQDSA
541GYNRPAHHMV PRPSVPPPGA NEEIPDDFDW DLIT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

574 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.