Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FOXD4L1 / FOXD5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114595-100
Catalog Number: LS-G114595
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FOXD4L1 (NM_012184), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA395L14.1 | FOXD5 | Forkhead box protein D4-like 1 | FOXD4-like 1 | Forkhead box D4 like 1 | Forkhead box D4-like 1 | FOXD4L1
- Origin Species
- Human
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MNLPRAERPR STPQRSLRDS DGEDGKIDVL GEEEDEDEVE DEEEEASQKF LEQSLQPGLQ61VARWGGVALP REHIEGGGPS DPSEFGTEFR APPRSAAASE DARQPAKPPY SYIALITMAI121LQSPHKRLTL SGICAFISGR FPYYRRKFPA WQNSIRHNLS LNDCFVKIPR EPGHPGKGTY181WSLDPASQDM FDNGSFLRRR KRFKRHQLTP GAHLPHPFPL PAAHAALHNP RPGPLLGAPA241LPQPVPGAYP NTAPGRRPYA LLHPHPPRYL LLSAPAYAGA PKKAEGADLA TPGTLPVLQP301SLGPQPWEEG KGLASPPGGG CISFSIESIM QGVRGAGTGA AQSLSPTAWS YCPLLQRPSS361LSDNFAATAA ASGGGLRQRL RSHQGRGAGR APVGRVGAAA VSGGGRGLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
408 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.