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FOXD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FOXD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G116615-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FOXD2 (NM_004474), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Forkhead box protein D2 | Fox d2 | Forkhead box D2 | Forkhead-like 17 | FREAC-9 | FREAC9 | FKHL17 | Forkhead related protein 17 | Forkhead-related activator 9 | FOXD2
Origin Species
Human
NCBI GenBank
NM_004474
SwissProt
O60548

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
48.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTLGSCCCEI MSSESSPAAL SEADADIDVV GGGSGGGELP ARSGPRAPRD VLPHGHEPPA
61EEAEADLAED EEESGGCSDG EPRALASRGA AAAAGSPGPG AAAARGAAGP GPGPPSGGAA
121TRSPLVKPPY SYIALITMAI LQSPKKRLTL SEICEFISGR FPYYREKFPA WQNSIRHNLS
181LNDCFVKIPR EPGNPGKGNY WTLDPESADM FDNGSFLRRR KRFKRQPLPP PHPHPHPHPE
241LLLRGGAAAA GDPGAFLPGF AAYGAYGYGY GLALPAYGAP PPGPAPHPHP HPHAFAFAAA
301AAAAPCQLSV PPGRAAAPPP GPPTASVFAG AGSAPAPAPA SGSGPGPGPA GLPAFLGAEL
361GCAKAFYPAS LSPPAAGTAA GLPTALLRQG LKTDAGGGAG GGGAGAGQRP SFSIDHIMGH
421GGGGAAPPGA GEGSPGPPFA AAAGPGGQAQ VLAMLTAPAL APVAGHIRLS HPGDALLSSG
481SRFASKVAGL SGCHF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

495 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.