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FNIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FNIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110947-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FNIP2 (NM_020840), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FNIP1-like protein | FNIPL | MAPO1 | FNIP2 | KIAA1450
Origin Species
Human
SwissProt
Q9P278

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
121.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPTLLQKLF NKRGSSGSSA AASAQGRAPK EGPAFSWSCS EFDLNEIRLI VYQDCDRRGR
61QVLFDSKAVQ KIEEVTAQKT EDVPIKISAK CCQGSSSVSS SSSSSISSHS SSGGSSHHAK
121EQLPKYQYTR PASDVNMLGE MMFGSVAMSY KGSTLKIHYI RSPPQLMISK VFSARMGSFC
181GSTNNLQDSF EYINQDPNLG KLNTNQNSLG PCRTGSNLAH STPVDMPSRG QNEDRDSGIA
241RSASLSSLLI TPFPSPSSST SSSSSYQRRW LRSQTTSLEN GIIPRRSTDE TFSLAEETCS
301SNPAMVRRKK IAISIIFSLC EKEEAQRNFQ DFFFSHFPLF ESHMNRLKSA IEKAMISCRK
361IAESSLRVQF YVSRLMEALG EFRGTIWNLY SVPRIAEPVW LTMMSGTLEK NQLCQRFLKE
421FTLLIEQINK NQFFAALLTA VLTYHLAWVP TVMPVDHPPI KAFSEKRTSQ SVNMLAKTHP
481YNPLWAQLGD LYGAIGSPVR LTRTVVVGKQ KDLVQRILYV LTYFLRCSEL QENQLTWSGN
541HGEGDQVLNG SKIITALEKG EVEESEYVVI TVRNEPALVP PILPPTAAER HNPWPTGFPE
601CPEGTDSRDL GLKPDKEANR RPEQGSEACS AGCLGPASDA SWKPQNAFCG DEKNKEAPQD
661GSSRLPSCEV LGAGMKMDQQ AVCELLKVEM PTRLPDRSVA WPCPDRHLRE KPSLEKVTFQ
721IGSFASPESD FESRMKKMEE RVKACGPSLE ASEAADVAQD PQVSRSPFKP GFQENVCCPQ
781NRLSEGDEGE SDKGFAEDRG SRNDMAADIA GQLSHAADLG TASHGAGGTG GRRLEATRGL
841YVKAAEGPVL EPVAPRCVQR GPGLVAGANI PCGDDNKKAN FRTEGDIPRN ESSDSALGDS
901DDEACASAML DLGHGGDRTG GSLEVELPLP RSQSISTQNV RNFGRSLLAG YCPTYMPDLV
961LHGTGSDEKL KQCLVADLVH TVHHPVLDEP IAEAVCIIAD TDKWSVQVAT SQRKVTDNMK
1021LGQDVLVSSQ VSSLLQSILQ LYKLHLPADF CIMHLEDRLQ EMYLKSKMLS EYLRGHTRVH
1081VKELGVVLGI ESNDLPLLTA IASTHSPYVA QILL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1114 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.