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FMNL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FMNL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103541-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FMNL3 (NM_198900), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Formin-like 3 | KIAA2014 | WBP3 | WW domain binding protein 3 | WW domain-binding protein 3 | FMNL3 | Formin-like protein 3 | WBP-3
Origin Species
Human
SwissProt
Q8IVF7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGNLESAEGV PGEPPSVPLL LPPGKMPMPE PCELEERFAL VLSSMNLPPD KARLLRQYDN
61EKKWDLICDQ ERFQVKNPPH TYIQKLQSFL DPSVTRKKFR RRVQESTKVL RELEISLRTN
121HIGWVREFLN DENKGLDVLV DYLSFAQCSV MYSTLPGRRA LKNSRLVSQK DDVHVCILCL
181RAIMNYQYGF NLVMSHPHAV NEIALSLNNK NPRTKALVLE LLAAVCLVRG GHEIILAAFD
241NFKEVCKELH RFEKLMEYFR NEDSNIDFMV ACMQFINIVV HSVEDMNFRV HLQYEFTKLG
301LEEFLQKSRH TESEKLQVQI QAYLDNVFDV GGLLEDAETK NVALEKVEEL EEHVSHLTEK
361LLDLENENMM RVAELEKQLL QREKELESIK ETYENTSHQV HTLRRLIKEK EEAFQRRCHL
421EPNVRGLESV DSEALARVGP AELSEGMPPS DLDLLAPAPP PEEVLPLPPP PAPPLPPPPP
481PLPDKCPPAP PLPGAAPSVV LTVGLSAIRI KKPIKTKFRL PVFNWTALKP NQISGTVFSE
541LDDEKILEDL DLDKFEELFK TKAQGPALDL ICSKNKTAQK AASKVTLLEA NRAKNLAITL
601RKAGRSAEEI CRAIHTFDLQ TLPVDFVECL MRFLPTEAEV KLLRQYERER QPLEELAAED
661RFMLLFSKVE RLTQRMAGMA FLGNFQDNLQ MLTPQLNAII AASASVKSSQ KLKQMLEIIL
721ALGNYMNSSK RGAVYGFKLQ SLDLLLDTKS TDRKMTLLHF IALTVKEKYP DLANFWHELH
781FVEKAAAVSL ENVLLDVKEL GRGMELIRRE CSIHDNSVLR NFLSTNEGKL DKLQRDAKTA
841EEAYNAVVRY FGESPKTTPP SVFFPVFVRF IRSYKEAEQE NEARKKQEEV MREKQLAQEA
901KKLDAKTPSQ RNKWQQQELI AELRRRQAKE HRPVYEGKDG TIEDIITGLH CQPMVVRHQA
961RSAAPPSGPP RAPGPH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

976 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.