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FMNL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FMNL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108104-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FMNL2 (NM_052905), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FMNL2 | Formin-like protein 2 | FHOD2 | KIAA1902 | Formin-like 2
Origin Species
Human
SwissProt
Q96PY5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGNAGSMDSQ QTDFRAHNVP LKLPMPEPGE LEERFAIVLN AMNLPPDKAR LLRQYDNEKK
61WELICDQERF QVKNPPHTYI QKLKGYLDPA VTRKKFRRRV QESTQVLREL EISLRTNHIG
121WVREFLNEEN KGLDVLVEYL SFAQYAVTFD FESVESTVES SVDKSKPWSR SIEDLHRGSN
181LPSPVGNSVS RSGRHSALRY NTLPSRRTLK NSRLVSKKDD VHVCIMCLRA IMNYQYGFNM
241VMSHPHAVNE IALSLNNKNP RTKALVLELL AAVCLVRGGH EIILSAFDNF KEVCGEKQRF
301EKLMEHFRNE DNNIDFMVAS MQFINIVVHS VEDMNFRVHL QYEFTKLGLD EYLDKLKHTE
361SDKLQVQIQA YLDNVFDVGA LLEDAETKNA ALERVEELEE NISHLSEKLQ DTENEAMSKI
421VELEKQLMQR NKELDVVREI YKDANTQVHT LRKMVKEKEE AIQRQSTLEK KIHELEKQGT
481IKIQKKGDGD IAILPVVASG TLSMGSEVVA GNSVGPTMGA ASSGPLPPPP PPLPPSSDTP
541ETVQNGPVTP PMPPPPPPPP PPPPPPPPPP PPPLPGPAAE TVPAPPLAPP LPSAPPLPGT
601SSPTVVFNSG LAAVKIKKPI KTKFRMPVFN WVALKPNQIN GTVFNEIDDE RILEDLNVDE
661FEEIFKTKAQ GPAIDLSSSK QKIPQKGSNK VTLLEANRAK NLAITLRKAG KTADEICKAI
721HVFDLKTLPV DFVECLMRFL PTENEVKVLR LYERERKPLE NLSDEDRFMM QFSKIERLMQ
781KMTIMAFIGN FAESIQMLTP QLHAIIAASV SIKSSQKLKK ILEIILALGN YMNSSKRGAV
841YGFKLQSLDL LLDTKSTDRK QTLLHYISNV VKEKYHQVSL FYNELHYVEK AAAVSLENVL
901LDVKELQRGM DLTKREYTMH DHNTLLKEFI LNNEGKLKKL QDDAKIAQDA FDDVVKYFGE
961NPKTTPPSVF FPVFVRFVKA YKQAEEENEL RKKQEQALME KLLEQEALME QQDPKSPSHK
1021SKRQQQELIA ELRRRQVKDN RHVYEGKDGA IEDIITDLRN QPYRRADAVR RSVRRRFDDQ
1081NLRSVNGAEI TM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1092 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.