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FMN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FMN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FMN1 antibodies →
$725.00each
Catalog Number: LS-G118851-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FMN1 (NM_001103184), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENVDNSLDG SDVSEPAKPE AGLEVAQSIL SKFSMKSLFG FTSKLESVNP EEEDAVLKAF
61HSLDVNPTSQ QDDSSNGLDP QEAGSRVSPD LGNDEKIASV ETESEGSQRK EAGTSLLAQE
121LLPLSTLKGT KDDVICVRGT LVHTTSDSDS DDGGQEPEEG SSTNGPKSPS GVLSEPSQES
181KENPGGFREN TVTGEMNGAE LCAEDPQRIP PEMSSKLEAG NGGLQTERRP SQDQVGEEGS
241QDLPAVTNQN SSVGITESAS SKKEVSGEKS FQLPAFFSGL RVLKKGATAE GGETITEIKP
301KDGDLALLKL TQPVQKSLVQ AGLQTVKSEK KATDPKATPT LLEQLSLLLN IDMPKTEPKG
361ADPESPRREE MGCNADQESQ SGPGVPQTQG GEVKPKSPET ALEAFKALFI RPPRKGTTAD
421TSELEALKRK MRHEKESLRA VFERSNSKPA DGPSDSKSPD HSLTEQDDRT PGRLQAVWPP
481PKTKDTEEKV GLKYTEAEYQ AAILHLKREH KEEIENLQAQ FELRAFHIRG EHAMITARLE
541ETIENLKHEL EHRWRGGCEE RKDVCISTDD DCPPKTFRNV CVQTDRETFL KPCESESKTT
601RSNQLVPKKL NISSLSQLSP PNDHKDIHAA LQPMEGMASN QQKALPPPPA SIPPPPPLPS
661GLGSLSPAPP MPPVSAGPPL PPPPPPPPPL PPPSSAGPPP PPPPPPLPNS PAPPNPGGPP
721PAPPPPGLAP PPPPGLFFGL GSSSSQCPRK PAIEPSCPMK PLYWTRIQIS DRSQNATPTL
781WDSLEEPDIR DPSEFEYLFS KDTTQQKKKP LSETYEKKNK VKKIIKLLDG KRSQTVGILI
841SSLHLEMKDI QQAIFNVDDS VVDLETLAAL YENRAQEDEL VKIRKYYETS KEEELKLLDK
901PEQFLHELAQ IPNFAERAQC IIFRSVFSEG ITSLHRKVEI ITRASKDLLH VKSVKDILAL
961ILAFGNYMNG GNRTRGQADG YSLEILPKLK DVKSRDNGIN LVDYVVKYYL RYYDQEAGTE
1021KSVFPLPEPQ DFFLASQVKF EDLIKDLRKL KRQLEASEKQ MVVVCKESPK EYLQPFKDKL
1081EEFFQKAKKE HKMEESHLEN AQKSFETTVR YFGMKPKSGE KEITPSYVFM VWYEFCSDFK
1141TIWKRESKNI SKERLKMAQE SVSKLTSEKK VETKKINPTA SLKERLRQKE ASVTTN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1196 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.