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FLRT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FLRT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FLRT1 antibodies →
$504.00each
Catalog Number: LS-G114355-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FLRT1 (NM_013280), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FLRT1
Origin Species
Human
NCBI GenBank
NM_013280
SwissProt
Q9NZU1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVVAHPTATA TTTPTATVTA TVVMTTATMD LRDWLFLCYG LIAFLTEVID STTCPSVCRC
61DNGFIYCNDR GLTSIPADIP DDATTLYLQN NQINNAGIPQ DLKTKVNVQV IYLYENDLDE
121FPINLPRSLR ELHLQDNNVR TIARDSLARI PLLEKLHLDD NSVSTVSIEE DAFADSKQLK
181LLFLSRNHLS SIPSGLPHTL EELRLDDNRI STIPLHAFKG LNSLRRLVLD GNLLANQRIA
241DDTFSRLQNL TELSLVRNSL AAPPLNLPSA HLQKLYLQDN AISHIPYNTL AKMRELERLD
301LSNNNLTTLP RGLFDDLGNL AQLLLRNNPW FCGCNLMWLR DWVKARAAVV NVRGLMCQGP
361EKVRGMAIKD ITSEMDECFE TGPQGGVANA AAKTTASNHA SATTPQGSLF TLKAKRPGLR
421LPDSNIDYPM ATGDGAKTLA IHVKALTADS IRITWKATLP ASSFRLSWLR LGHSPAVGSI
481TETLVQGDKT EYLLTALEPK STYIICMVTM ETSNAYVADE TPVCAKAETA DSYGPTTTLN
541QEQNAGPMAS LPLAGIIGGA VALVFLFLVL GAICWYVHQA GELLTRERAY NRGSRKKDDY
601MESGTKKDNS ILEIRGPGLQ MLPINPYRAK EEYVVHTIFP SNGSSLCKAT HTIGYGTTRG
661YRDGGIPDID YSYT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

674 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.