LSBio
FLJ22028 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FLJ22028 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PYROXD1 antibodies →
$504.00each
Catalog Number: LS-G109711-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PYROXD1 (NM_024854), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PYROXD1 | FLJ22028
Origin Species
Human
NCBI GenBank
NM_024854
SwissProt
Q8WU10

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MEAARPPPTA GKFVVVGGGI AGVTCAEQLA THFPSEDILL VTASPVIKAV TNFKQISKIL
61EEFDVEEQSS TMLGKRFPNI KVIESGVKQL KSEEHCIVTE DGNQHVYKKL CLCAGAKPKL
121ICEGNPYVLG IRDTDSAQEF QKQLTKAKRI MIIGNGGIAL ELVYEIEGCE VIWAIKDKAI
181GNTFFDAGAA EFLTSKLIAE KSEAKIAHKR TRYTTEGRKK EARSKSKADN VGSALGPDWH
241EGLNLKGTKE FSHKIHLETM CEVKKIYLQD EFRILKKKSF TFPRDHKSVT ADTEMWPVYV
301ELTNEKIYGC DFIVSATGVT PNVEPFLHGN SFDLGEDGGL KVDDHMHTSL PDIYAAGDIC
361TTSWQLSPVW QQMRLWTQAR QMGWYAAKCM AAASSGDSID MDFSFELFAH VTKFFNYKVV
421LLGKYNAQGL GSDHELMLRC TKGREYIKVV MQNGRMMGAV LIGETDLEET FENLILNQMN
481LSSYGEDLLD PNIDIEDYFD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

500 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.