Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FLJ12529 / CPSF7 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109755-100
Catalog Number: LS-G109755
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CPSF7 (NM_024811), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CFIm59 | CPSF7 | CPSF 59 kDa subunit | FLJ12529
- Origin Species
- Human
- NCBI GenBank
- NM_024811
- SwissProt
- Q8N684
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRPESAGGG SRGPFEGGGR ARRAGGIFLT LSILRTRDLP SGAMSEGVDL IDIYADEEFN61QDPEFNNTDQ IDLYDDVLTA TSQPSDDRSS STEPPPPVRQ EPSPKPNNKT PAILYTYSGL121RNRRAAVYVG SFSWWTTDQQ LIQVIRSIGV YDVVELKFAE NRANGQSKGY AEVVVASENS181VHKLLELLPG KVLNGEKVDV RPATRQNLSQ FEAQARKREC VRVPRGGIPP RAHSRDSSDS241ADGRATPSEN LVPSSARVDK PPSVLPYFNR PPSALPLMGL PPPPIPPPPP LSSSFGVPPP301PPGIHYQHLM PPPPRLPPHL AVPPPGAIPP ALHLNPAFFP PPNATVGPPP DTYMKASAPY361NHHGSRDSGP PPSTVSEAEF EDIMKRNRAI SSSAISKAVS GASAGDYSDA IETLLTAIAV421IKQSRVANDE RCRVLISSLK DCLHGIEAKS YSVGASGSSS RKRHRSRERS PSRSRESSRR481HRDLLHNEDR HDDYFQERNR EHERHRDRER DRHHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
514 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.