Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FKBP4 / FKBP52 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118239-100
Catalog Number: LS-G118239
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FKBP52(FKBP4) (NM_002014), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 59 kDa immunophilin | 51 kDa FK506-binding protein | 52 kDa FK506-binding protein | 52 kDa FKBP | FKBP-52 | FKBP4 | FKBP51 | HBI | Immunophilin FKBP52 | FK506 binding protein 4 | 59kDa | FK506-binding protein 4 | HSP56 | p52 | p59 | Rotamase | p59 protein | PPIase | PPIase FKBP4 | FKBP-4 | FKBP52 | FKBP59 | HSP binding immunophilin | HSP-binding immunophilin
- Origin Species
- Human
- NCBI GenBank
- NM_002014
- SwissProt
- Q02790
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW61LLDGTKFDSS LDRKDKFSFD LGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP121KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKD181KLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN241AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVY FKEGKYKQAL LQYKKIVSWL301EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG361EAHLAVNDFE LARADFQKVL QLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE421EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEAT RTRPLEQKLI SEEDLAANDI481LDYKDDDDKV
490 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.