Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FKBP25 / FKBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99620-100
Catalog Number: LS-G99620
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FKBP25(FKBP3) (NM_002013), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 25 kDa FK506-binding protein | 25 kDa FKBP | FKBP3 | FKBP-3 | Immunophilin FKBP25 | FK506-binding protein 3 | FK506-binding protein 3 (25kD) | PPIase FKBP3 | Rapamycin binding protein | Rotamase | PPIase | FK506 binding protein 3 | 25kDa | FKBP-25 | FKBP25
- NCBI GenBank
- NM_002013
- SwissProt
- Q00688
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 25 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV61TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD121KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL181TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDIDTRTRPL EQKLISEEDL241AANDILDYKD DDDKV
255 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.