Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FKBP1A / FKBP12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108005-100
Catalog Number: LS-G108005
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FKBP12(FKBP1A) (NM_054014), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 12 kDa FKBP | 12 kDa FK506-binding protein | Calstabin-1 | FK506 binding protein12 | Fk506-binding protein | FKBP12C | FK506-binding protein 12 | FKBP-1A | Immunophilin FKBP12 | FK506-binding protein 1 | FKBP1 | FKBP12-Exip3 | FKBP1A | PKC12 | PKCI2 | PPIase FKBP1A | Protein kinase C inhibitor 2 | Rotamase | PPIase | FK506-binding protein 1A | FKBP-12
- Origin Species
- Human
- NCBI GenBank
- NM_000801
- SwissProt
- P62942
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 11.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW61EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLETR TRPLEQKLIS121EEDLAANDIL DYKDDDDKV
139 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.