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FIZ1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FIZ1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102093-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FIZ1 (NM_032836), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FLT3-interacting zinc finger 1 | Zinc finger protein 798 | FIZ1 | ZNF798
Origin Species
Human
NCBI GenBank
NM_032836
SwissProt
Q96SL8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MDDVPAPTPA PAPPAAAAPR VPFHCSECGK SFRYRSDLRR HFARHTALKP HACPRCGKGF
61KHSFNLANHL RSHTGERPYR CSACPKGFRD STGLLHHQVV HTGEKPYCCL VCELRFSSRS
121SLGRHLKRQH RGVLPSPLQP GPGLPALSAP CSVCCNVGPC SVCGGSGAGG GEGPEGAGAG
181LGSWGLAEAA AAAAASLPPF ACGACARRFD HGRELAAHWA AHTDVKPFKC PRCERDFNAP
241ALLERHKLTH DLQGPGAPPA QAWAAGPGAG PETAGEGTAA EAGDAPLASD RRLLLGPAGG
301GVPKLGGLLP EGGGEAPAPA AAAEPSEDTL YQCDCGTFFA SAAALASHLE AHSGPATYGC
361GHCGALYAAL AALEEHRRVS HGEGGGEEAA AAAREREPAS GEPPSGSGRG KKIFGCSECE
421KLFRSPRDLE RHVLVHTGEK PFPCLECGKF FRHECYLKRH RLLHGTERPF PCHICGKGFI
481TLSNLSRHLK LHRGMD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

496 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.