Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FHOD3 / FHOS2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109567-100
Catalog Number: LS-G109567
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FHOD3 (NM_025135), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FHOS2 | Formactin2 | HFHOS2 | FHOD3 | Formactin-2 | KIAA1695
- Origin Species
- Human
- NCBI GenBank
- AK025950
- SwissProt
- Q2V2M9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 160.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATLACRVQF LDDTDPFNST NFPEPSRPPL FTFREDLALG TQLAGVHRLL QAPHKLDDCT61LQLSHNGAYL DLEATLAEQR DELEGFQDDA GRGKKHSIIL RTQLSVRVHA CIEKLYNSSG121RDLRRALFSL KQIFQDDKDL VHEFVVAEGL TCLIKVGAEA DQNYQNYILR ALGQIMLYVD181GMNGVINRNE TIQWLYTLIG SKFRLVVKTA LKLLLVFVEY SESNAPLLIQ AVTAVDTKRG241VKPWSNIMEI LEEKDGVDTE LLVYAMTLVN KTLSGLPDQD TFYDVVDCLE ELGIAAVSQR301HLNKKGTDLD LVEQLNIYEV ALRHEDGDET TEPPPSGCRD RRRASVCSSG GGEHRGLDRR361RSRRHSVQSI KSTLSAPTSP CSQSAPSFKP NQVRDLREKY SNFGNNSYHS SRPSSGSSVP421TTPTSSVSPP QEARLERSSP SGLLTSSFRQ HQESLAAERE RRRQEREERL QRIEREERNK481FSRDYLDKRE EQRQAREERY KYLEQLAAEE HEKELRSRSV SRGRADLSLD LTSPAAPACL541APLSHSPSSS DSQEALTVSA SSPGTPHHPQ ASAGDPEPES EAEPEAEAGA GQVADEAGQD601IASAHEGAET EVEQALEQEP EERASLSEKE RQNEGVNERD NCSASSVSSS SSTLEREEKE661DKLSRDRTTG LWPAGVQDAG VNGQCGDILT NKRFMLDMLY AHNRKSPDDE EKGDGEAGRT721QQEAEAVASL ATRISTLQAN SQTQDESVRR VDVGCLDNRG SVKAFAEKFN SGDLGRGSIS781PDAEPNDKVP ETAPVQPKTE SDYIWDQLMA NPRELRIQDM DFTDLGEEDD IDVLDVDLGH841REAPGPPPPP PPTFLGLPPP PPPPLLDSIP PPPVPGNLLV PPPPVFNAPQ GLGWSQVPRG901QPTFTKKKKT IRLFWNEVRP FDWPCKNNRR CREFLWSKLE PIKVDTSRLE HLFESKSKEL961SVSKKTAADG KRQEIIVLDS KRSNAINIGL TVLPPPRTIK IAILNFDEYA LNKEGIEKIL1021TMIPTDEEKQ KIQEAQLANP EIPLGSAEQF LLTLSSISEL SARLHLWAFK MDYETTEKEV1081AEPLLDLKEG IDQLENNKTL GFILSTLLAI GNFLNGTNAK AFELSYLEKV PEVKDTVHKQ1141SLLHHVCTMV VENFPDSSDL YSEIGAITRS AKVDFDQLQD NLCQMERRCK ASWDHLKAIA1201KHEMKPVLKQ RMSEFLKDCA ERIIILKIVH RRIINRFHSF LLFMGHPPYA IREVNINKFC1261RIISEFALEY RTTRERVLQQ KQKRANHRER NKTRGKMITD SGKFSGSSPA PPSQPQGLSY1321AEDAAEHENM KAVLKTSSPS VEDATPALGV RTRSRASRGS TSSWTMGTDD SPNVTDDAAD1381EIMDRIVKSA TQVPSQRVVP RERKRSRANR KSLRRTLKSG LTPEEARALG LVGTSELQLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1439 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.