LSBio
FHDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FHDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FHDC1 antibodies →
$725.00each
Catalog Number: LS-G108272-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FHDC1 (NM_033393), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_033393

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHVMNCVSLV SDKENGNIAT APGFMIGQTP PPAPPPPPPP PPPSPPCSCS REECPSSPPP
61PPPPPLPGEP PIPPPPPGLP PTTHMNGYSH LGKKKRMRSF FWKTIPEEQV RGKTNIWTLA
121ARQEHHYQID TKTIEELFGQ QEDTTKSSLP RRGRTLNSSF REAREEITIL DAKRSMNIGI
181FLKQFKKSPR SIVEDIHQGK SEHYGSETLR EFLKFLPESE EVKKLKAFSG DVSKLSLADS
241FLYGLIQVPN YSLRIEAMVL KKEFLPSCSS LYTDITVLRT AIKELMSCEE LHSILHLVLQ
301AGNIMNAGGY AGNAVGFKLS SLLKLADTKA NKPGMNLLHF VAQEAQKKDT ILLNFSEKLH
361HVQKTARLSL ENTEAELHLL FVRTKSLKEN IQRDGELCQQ MEDFLQFAIE KLRELECWKQ
421ELQDEAYTLI DFFCEDKKTM KLDECFQIFR DFCTKFNKAV KDNHDREAQE LRQLQRLKEQ
481EQKQRSWATG ELGAFGRSSS ENDVELLTKK GAEGLLPFLH PRPISPSSPS YRPPNTRRSR
541LSLGPSADRE LLTFLESSTG SPEEPNKFHS LPRSSPRQAR PTIACLEPAE VRHQDSSFAH
601KPQASGGQEE APNPPSAQAH QLAAAQPENH ASAFPRARCQ GVSVLRKRYS EPVSLGSAQS
661PPLSPLALGI KEHELVTGLA QFNLQGSQGM EETSQLTLSD FSPMELESVG HRGPQSLSAS
721SSSLTPMGRD ALGSLSPALE DGKAAPDEPG SAALGSVGSS DPENKDPRPL FCISDTTDCS
781LTLDCSEGTD SRPRGGDPEE GGEGDGSMSS GVGEMGDSQV SSNPTSSPPG EAPAPVSVDS
841EPSCKGGLPR DKPTKRKDVV APKRGSLKEA SPGASKPGSA RRSQGAVAKS VRTLTASENE
901SMRKVMPITK SSRGAGWRRP ELSSRGPSQN PPSSTDTVWS RQNSVRRAST GAEEQRLPRG
961SSGSSSTRPG RDVPLQPRGS FKKPSAKPLR NLPRQKPEEN KTCRAHSEGP ESPKEEPKTP
1021SVPSVPHELP RVPSFARNTV ASSSRSMRTD LPPVAKAPGI TRTVSQRQLR VKGDPEDAAP
1081KDSSTLRRAS SARAPKKRPE SAEGPSANTE APLKARGAGE RASLRRKDSS RTTLGRILNP
1141LRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1143 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.