Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FGL1 / Hepassocin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103230-100
Catalog Number: LS-G103230
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FGL1 (NM_201553), transcript variant 4 cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Fibrinogen-like 1 | FGL1 | Hepassocin | HFREP-1 | HFREP1 | HP-041 | LFIRE-1 | Fibrinogen-like protein 1 | LFIRE1
- Origin Species
- Human
- NCBI GenBank
- NM_004467
- SwissProt
- Q08830
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAKVFSFILV TTALTMGREI SALEDCAQEQ MRLRAQVRLL ETRVKQQQVK IKQLLQENEV61QFLDKGDENT VIDLGSKRQY ADCSEIFNDG YKLSGFYKIK PLQSPAEFSV YCDMSDGGGW121TVIQRRSDGS ENFNRGWKDY ENGFGNFVQK HGEYWLGNKN LHFLTTQEDY TLKIDLADFE181KNSRYAQYKN FKVGDEKNFY ELNIGEYSGT AGDSLAGNFH PEVQWWASHQ RMKFSTWDRD241HDNYEGNCAE EDQSGWWFNR CHSANLNGVY YSGPYTAKTD NGIVWYTWHG WWYSLKSVVM301KIRPNDFIPN VITRTRPLEQ KLISEEDLAA NDILDYKDDD DKV
343 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.