Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FGFR1 / FGF Receptor 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113047-100
Catalog Number: LS-G113047
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FGFR1 (NM_015850), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BFGF receptor | BFGF-R | BFGF-R-1 | CD331 | Cek1 | CD331 antigen | FGFR1 | FGFR1/PLAG1 fusion | FLT2 | Fgf-flg | FGFBR | FGFR-1 | Fms-like tyrosine kinase 2 | HBGFR | HH2 | Hydroxyaryl-protein kinase | FGF receptor 1 | Fms-related tyrosine kinase 2 | Proto-oncogene c-Fgr | BFGFR | CEK | FLT-2 | HBGF receptor | KAL2 | OGD
- Origin Species
- Human
- NCBI GenBank
- NM_023110
- SwissProt
- P11362
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.38 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWSWKCLLFW AVLVTATLCT ARPSPTLPEQ AQPWGAPVEV ESFLVHPGDL LQLRCRLRDD61VQSINWLRDG VQLAESNRTR ITGEEVEVQD SVPADSGLYA CVTSSPSGSD TTYFSVNVSD121ALPSSEDDDD DDDSSSEEKE TDNTKPNPVA PYWTSPEKME KKLHAVPAAK TVKFKCPSSG181TPNPTLRWLK NGKEFKPDHR IGGYKVRYAT WSIIMDSVVP SDKGNYTCIV ENEYGSINHT241YQLDVVERSP HRPILQAGLP ANKTVALGSN VEFMCKVYSD PQPHIQWLKH IEVNGSKIGP301DNLPYVQILK TAGVNTTDKE MEVLHLRNVS FEDAGEYTCL AGNSIGLSHH SAWLTVLEAL361EERPAVMTSP LYLEIIIYCT GAFLISCMVG SVIVYKMKSG TKKSDFHSQM AVHKLAKSIP421LRRQVSADSS ASMNSGVLLV RPSRLSSSGT PMLAGVSEYE LPEDPRWELP RDRLVLGKPL481GEGCFGQVVL AEAIGLDKDK PNRVTKVAVK MLKSDATEKD LSDLISEMEM MKMIGKHKNI541INLLGACTQD GPLYVIVEYA SKGNLREYLQ ARRPPGLEYC YNPSHNPEEQ LSSKDLVSCA601YQVARGMEYL ASKKCIHRDL AARNVLVTED NVMKIADFGL ARDIHHIDYY KKTTNGRLPV661KWMAPEALFD RIYTHQSDVW SFGVLLWEIF TLGGSPYPGV PVEELFKLLK EGHRMDKPSN721CTNELYMMMR DCWHAVPSQR PTFKQLVEDL DRIVALTSNQ EYLDLSMPLD QYSPSFPDTR781SSTCSSGEDS VFSHEPLPEE PCLPRHPAQL ANGGLKRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
818 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.