Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FGFR1 / FGF Receptor 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110203-100
Catalog Number: LS-G110203
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FGFR1 (NM_023106), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BFGF receptor | BFGF-R | BFGF-R-1 | CD331 | Cek1 | CD331 antigen | FGFR1 | FGFR1/PLAG1 fusion | FLT2 | Fgf-flg | FGFBR | FGFR-1 | Fms-like tyrosine kinase 2 | HBGFR | HH2 | Hydroxyaryl-protein kinase | FGF receptor 1 | Fms-related tyrosine kinase 2 | Proto-oncogene c-Fgr | BFGFR | CEK | FLT-2 | HBGF receptor | KAL2 | OGD
- Origin Species
- Human
- NCBI GenBank
- NM_023110
- SwissProt
- P11362
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWSWKCLLFW AVLVTATLCT ARPSPTLPEQ DALPSSEDDD DDDDSSSEEK ETDNTKPNPV61APYWTSPEKM EKKLHAVPAA KTVKFKCPSS GTPNPTLRWL KNGKEFKPDH RIGGYKVRYA121TWSIIMDSVV PSDKGNYTCI VENEYGSINH TYQLDVVERS PHRPILQAGL PANKTVALGS181NVEFMCKVYS DPQPHIQWLK HIEVNGSKIG PDNLPYVQIL KTAGVNTTDK EMEVLHLRNV241SFEDAGEYTC LAGNSIGLSH HSAWLTVLEA LEERPAVMTS PLYLEIIIYC TGAFLISCMV301GSVIVYKMKS GTKKSDFHSQ MAVHKLAKSI PLRRQVTVSA DSSASMNSGV LLVRPSRLSS361SGTPMLAGVS EYELPEDPRW ELPRDRLVLG KPLGEGCFGQ VVLAEAIGLD KDKPNRVTKV421AVKMLKSDAT EKDLSDLISE MEMMKMIGKH KNIINLLGAC TQDGPLYVIV EYASKGNLRE481YLQARRPPGL EYCYNPSHNP EEQLSSKDLV SCAYQVARGM EYLASKKCIH RDLAARNVLV541TEDNVMKIAD FGLARDIHHI DYYKKTTNGR LPVKWMAPEA LFDRIYTHQS DVWSFGVLLW601EIFTLGGSPY PGVPVEELFK LLKEGHRMDK PSNCTNELYM MMRDCWHAVP SQRPTFKQLV661EDLDRIVALT SNQEYLDLSM PLDQYSPSFP DTRSSTCSSG EDSVFSHEPL PEEPCLPRHP721AQLANGGLKR RTRTRPLEQKLISEEDLAANDILDYKDDDDKV
731 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.