Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FGF1 / Acidic FGF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99162-100
Catalog Number: LS-G99162
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FGF1 (NM_000800), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AFGF | Endothelial cell growth factor | Fibroblast growth factor 1 | FGFA | ECGFB | HBGF-1 | FGF1 | GLIO703 | ECGF | ECGF-beta | ECGFA | FGF-1 | FGF-alpha | HBGF1
- NCBI GenBank
- X59065
- SwissProt
- P05230
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 17.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEGEITTFT ALTEKFNLPP GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ61LSAESVGEVY IKSTETGQYL AMDTDGLLYG SQTPNEECLF LERLEENHYN TYISKKHAEK121NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
155 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.