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FGD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FGD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FGD5 antibodies →
$725.00each
Catalog Number: LS-G129188-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FGD5 (NM_152536), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
SwissProt
Q6ZNL6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
159.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFRGPKPPIA PKPRLTAPNE WRASVYLNDS LNKCSNGRLP CVDRGLDEGP RSIPKCSESE
61TDEDYIVVPR VPLREDEPKD EGSVGNKALV SPESSAEEEE EREEGGEACG LEGTGAGEDS
121VAPAAPGAGA LSREGEEGTD LALEDEGEGC ADEPGTLEQV SRSEEEEKLV QPHRECSLED
181SGPWAGEGVF QSDLLLPHIH GEDQEPPDTP GEAEEDDEEG CASTDPAGAD EGSGPDRPTE
241DMGQDAEDTS EEPPEKEELA GVQEAETATD CPEVLEEGCE EATGVTGGEQ VDLSEPPDHE
301KKTNQEVAAA TLEDHAQDES AEESCQIVPF ENDCMEDFVT SLTGSPYEFF PTESTSFCSE
361SCSPLSESAK GLESEQAPKL GLRAEENPMV GALCGQCGSL QGGAAEGPAA PDVVVVLEEE
421ALDDALANPY VMGVGLPGQA APGEGGQAAS DALGGYGSKE ELNCEAEGGL VPADRKNTST
481RVRPHSGKVA GYVPETVPEE TGPEAGSSAP GIGGAAEEVG KTLLSLEGKP LEASRALPAK
541PRAFTLYPRS FSVEGREIPV SVYQEPEGSG LDDHRIKRKE DNLSLSCVIG SSGSFSQRNH
601LPSSGTSTPS SMVDIPPPFD LACITKKPIT KSSPSLLIES DSPDKYKKKK SSFKRFLALT
661FKKKTENKLH VDVNVSSSRS SSESSYHGPS RILEVDRRSL SNSPQLKSRT GKLRASESPS
721SLIFYRDGKR KGVPFSRTVS RVESFEDRSR PPFLPLPLTK PRSISFPSAD TSDYENIPAM
781NSDYENIQIP PRRPARAGAF TKLFEDQSRA LSTANENDGY VDMSSFNAFE SKQQSADQDA
841ESAYTEPYKV CPISSAAPKE DLTSDEEQRS SEEEDSASRD PSVTHKVEGQ SRALVIAQEL
901LSSEKAYVEM LQHLNLDFHG AVMRALDDMD HEGRDTLARE ELRQGLSELP AIHDLHQGIL
961EELEERLSNW ESQQKVADVF LAREQGFDHH ATHILQFDRY LGLLSENCLH SPRLAAAVRE
1021FEQSVQGGSQ TAKHRLLRVV QRLFQYQVLL TDYLNNLCPD SAEYDNTQGA LSLISKVTDR
1081ANDSMEQGEN LQKLVHIEHS VRGQGDLLQP GREFLKEGTL MKVTGKNRRP RHLFLMNDVL
1141LYTYPQKDGK YRLKNTLAVA NMKVSRPVME KVPYALKIET SESCLMLSAS SCAERDEWYG
1201CLSRALPEDY KAQALAAFHH SVEIRERLGV SLGERPPTLV PVTHVMMCMN CGCDFSLTLR
1261RHHCHACGKI VCRNCSRNKY PLKYLKDRMA KVCDGCFGEL KKRGRAVPGL MRERPVSMSF
1321PLSSPRFSGS AFSSVFQSIN PSTFKKQKKV PSALTEVAAS GEGSAISGYL SRCKRGKRHW
1381KKLWFVIKGK VLYTYMASED KVALESMPLL GFTIAPEKEE GSSEVGPIFH LYHKKTLFYS
1441FKAEDTNSAQ RWIEAMEDAS VL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1462 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.