Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FFAR3 / GPR41 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100517-100
Catalog Number: LS-G100517
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FFAR3 (NM_005304), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FFAR3 | G-protein coupled receptor 41 | FFA3R | Gpcr41 | Gpr42 | Free fatty acid receptor 3 | G protein-coupled receptor 41 | GPR41
- Origin Species
- Human
- NCBI GenBank
- NM_005304
- SwissProt
- O14843
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDTGPDQSYF SGNHWFVFSV YLLTFLVGLP LNLLALVVFV GKLQRRPVAV DVLLLNLTAS61DLLLLLFLPF RMVEAANGMH WPLPFILCPL SGFIFFTTIY LTALFLAAVS IERFLSVAHP121LWYKTRPRLG QAGLVSVACW LLASAHCSVV YVIEFSGDIS HSQGTNGTCY LEFRKDQLAI181LLPVRLEMAV VLFVVPLIIT SYCYSRLVWI LGRGGSHRRQ RRVAGLLAAT LLNFLVCFGP241YNVSHVVGYI CGESPAWRIY VTLLSTLNSC VDPFVYYFSS SGFQADFHEL LRRLCGLWGQ301WQQESSMELK EQKGGEEQRA DRPAERKTSE HSQGCGTGGQ VACAESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
346 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.