Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FEZL / FEZF2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112017-100
Catalog Number: LS-G112017
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF312(FEZF2) (NM_018008), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FEZ family zinc finger 2 | FEZL | FEZF2 | FKSG36 | TOF | ZNF312 | ZFP312 | Zinc finger protein 312 | Zinc finger protein Fez-like | FEZ
- Origin Species
- Human
- NCBI GenBank
- NM_018008
- SwissProt
- Q8TBJ5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSASLETM VPPACPRAGA SPATSKTLAF SIERIMAKTS EPRAPFEPRP GALEADGGQG61KKLLNLCSPL PCMIPLQPLG YEVPSKTLLS YSELWKSSLR AGGGGGGGGG GGGGGGGAPV121CGASGLCKTN CGVCCKAELG LAPSALPAGR VIKPQVINQA VGLPASGSLY YFNYLDSTAY181PPSELLSGHL FPSGLLNAQA PAALAAHPKL FLLENAKLAG LAADKFPHPA PYPHKERLPA241PLEQVLKENS ALTAERGGVK GHSKLPGGSA DGKPKNFTCE VCGKVFNAHY NLTRHMPVHT301GARPFVCKVC GKGFRQASTL CRHKIIHTQE KPHKCNQCGK AFNRSSTLNT HIRIHAGYKP361FVCEFCGKGF HQKGNYKNHK LTHSGEKQYK CTICNKAFHQ VYNLTFHMHT HNDKKPFTCA421TCGKGFCRNF DLKKHVRKLH DSVGPAAPSA KDLTRTVQSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
459 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.