Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FEZF1 / FEZ Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128993-100
Catalog Number: LS-G128993
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF312B(FEZF1) (NM_001024613), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FEZF1 | FEZ family zinc finger 1 | Zinc finger protein 312B | ZNF312B | FEZ
- Origin Species
- Human
- NCBI GenBank
- NM_001024613
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSSCHNATT KMLATAPARG NMMSTSKPLA FSIERIMART PEPKALPVPH FLQGALPKGE61PKHSLHLNSS IPCMIPFVPV AYDTSPKAGV TGSEPRKASL EAPAAPAAVP SAPAFSCSDL121LNCALSLKGD LARDALPLQQ YKLVRPRVVN HSSFHAMGAL CYLNRGDGPC HPAAGVNIHP181VASYFLSSPL HPQPKTYLAE RNKLVVPAVE KYPSGVAFKD LSQAQLQHYM KESAQLLSEK241IAFKTSDFSR GSPNAKPKVF TCEVCGKVFN AHYNLTRHMP VHTGARPFVC KVCGKGFRQA301STLCRHKIIH TQEKPHKCNQ CGKAFNRSST LNTHTRIHAG YKPFVCEFCG KGFHQKGNYK361NHKLTHSGEK QFKCNICNKA FHQVYNLTFH MHTHNDKKPF TCPTCGKGFC RNFDLKKHVR421KLHDSSLGLA RTPAGEPGTE PPPPLPQQPP MTLPPLQPPL PTPGPLQPGL HQGHQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
475 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.