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FES Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FES Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FES antibodies →
$725.00each
Catalog Number: LS-G126181-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FES (NM_001143783), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
c-fes | FES | FPS | Feline sarcoma oncogene | Proto-oncogene c-Fps | v-fps | p93c-fes | Proto-oncogene c-Fes | v-fes | v-fes feline sarcoma viral
Origin Species
Human
NCBI GenBank
NM_002005
SwissProt
P07332

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGFSSELCSP QGHGVLQQMQ EAELRLLEGM RKWMAQRVKS DREYAGLLHH MSLQDSGGQS
61RAISPDSPIS QTHSQDIEKL KSQYRALARD SAQAKRKYQE ASKDKDRDKA KDKYVRSLWK
121LFAHHNRYVL GVRAAQLHHQ HHHQLLLPGL LRSLQDLHEE MACILKEILQ EYLEISSLVQ
181DEVVAIHREM AAAAARIQPE AEYQGFLRQY GSAPDVPPCV TFDESLLEEG EPLEPGELQL
241NELTVESVQH TLTSVTDELA VATEMVFRRQ EMVTQLQQEL RNEEENTHPR ERVQLLGKRQ
301VLQEALQGLQ VALCSQAKLQ AQQELLQTKL EHLGPGEPPP VLLLQDDRHS TSSSEQEREG
361GRTPTLEILK SHISGIFRPK FSLPPPLQLI PEVQKPLHEQ LWYHGAIPRA EVAELLVHSG
421DFLVRESQGK QEYVLSVLWD GLPRHFIIQS LDNLYRLEGE GFPSIPLLID HLLSTQQPLT
481KKSGVVLHRA VPKDKWVLNH EDLVLGEQIG RGNFGEVFSG RLRADNTLVA VKSCRETLPP
541DLKAKFLQEA RILKQYSHPN IVRLIGVCTQ KQPIYIVMEL VQGGDFLTFL RTEGARLRVK
601TLLQMVGDAA AGMEYLESKC CIHRDLAARN CLVTEKNVLK ISDFGMSREE ADGVYAASGG
661LRQVPVKWTA PEALNYGRYS SESDVWSFGI LLWETFSLGA SPYPNLSNQQ TREFVEKGGR
721LPCPELCPDA VFRLMEQCWA YEPGQRPSFS TIYQELQSIR KRHR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

764 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.