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FER1L6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FER1L6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FER1L6 antibodies →
$725.00each
Catalog Number: LS-G120569-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FER1L6 (NM_001039112), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001039112

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
209.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFGLKVKKKR NKAEKGLILA NKAAKDSQGD TEALQEEPSH QEGPRGDLVH DDASIFPVPS
61ASPKRRSKLL TKIHDGEVRS QNYQIAITIT EARQLVGENI DPVVTIEIGD EKKQSTVKEG
121TNSPFYNEYF VFDFIGPQVH LFDKIIKISV FHHKLIGSVL IGSFKVDLGT VYNQPGHQFC
181NKWALLTDPG DIRTGTKGYL KCDISVMGKG DVLKTSPKTS DTEEPIEKNL LIPNGFPLER
241PWARFYVRLY KAEGLPKMNS SIMANVTKAF VGDSKDLVDP FVEVSFAGQM GRTTVQKNCA
301DPVWHEQVIF KEMFPPLCRR VKIQVWDEGS MNDVALATHF IDLKKISNEQ DGDKGFLPTF
361GPAWINLYGS PRNHSLMDDY QEMNEGFGEG VSFRGRILVE IAVEILSGRA QESKFSKALK
421ELKLPSKDKD SKSSKGKDKA DKTEDGKSQQ ASNKTNSTEV EVESFDVPPE IVPEKNEEFL
481LFGAFFEATM IDRKIGDKPI SFEVSIGNFG NLIDGGSHYG SKKSAESAEE DLLPLLHEGQ
541GDVAHDVPIP MASTTHPEKP LVTEGNRNYN YLPFEAKKPC VYFISSWGDQ TFRLHWSNML
601EKMADFLEES IEEVRELIKI SQEAPEEKMK TVLSDFISRS SAFISEAEKK PKMLNQTTLD
661KKRLTLCWQE LEAMCKEAKG IIQQQKKKLS VDEMIHEAQN FVEKIRFLVD EPQHTIPDVF
721IWMLSNNRRV AYARIASKDL LYSPVAGQMG KHCGKIKTHF LKPPGKRPAG WSVQAKVDVY
781LWLGSIKHAS AILDNLPVGY EAEMSSKGAG TNHPPSNLLY QEQHVFQLRA HMYQARGLIA
841ADSNGLSDPF AKVTFLSHCQ TTKIISQTLS PTWNQMLLFN DLVLHGDVKE LAESPPLVVV
901ELYDSDAVGK PEYLGATVAA PVVKLADQDY EPPRLCYHPI FCGNLSGGDL LAVFELLQVP
961PSGLQGLPPV EPPDITQIYP VPANIRPVLS KYRVEVLFWG VREMKKVQLL SVDRPQALIE
1021CGGQGVKSCV IQSYKNNPNF SIQADAFEVE LPENELLHPP LSICVVDWRA FGRSTLVGTY
1081TINYLKQFLC KLREPLAPIT QVDGTQPGHD ISDSLTATES SGAHSSSQDP PADHIYVDVE
1141PPPTVVPDSA QAQPAILVDV PDSSPMLEPE HTPVAQEPPK DGKPKDPRKP SRRSTKRRKR
1201TIADESAENV IDWWSKYYAS LKKAQKAKER NPKGKKGNTE AKPDEVVVDI EDGPKKKKDK
1261MLKKKPKDDG IPNLAILQIY DGDLESEFNN FEDWVKTFEL FRGKSTEDDH GLDGDXVIGK
1321FKGSFCIYKS PQDSSSEDSG QLRIQQGIPP NHPVTVLIRV YIVAAFNLSP ADPDGKSDPY
1381IVIKLGKTEI KDRDKYIPKQ LNPVFGRSFE IQATFPKESL LSILIYDHDM IGTDDLIGET
1441KIDLENRFYS KHRAICGLQS QYEIEGYNAW RDTSKPTEIL TKLCKDNKLD GPYFHPGKIQ
1501IGNQVFSGKT IFTEEDTDET VESYEHLALK VLHSWEDIPE VGCRLVPEHI ETRPLYHKDK
1561PGMEQGRLQM WVDMFPKDMP QPGPPVDISP RRPKGYELRV TIWNTEDVIL EDENIFTGQK
1621SSDIYVKGWL KGLEDDKQET DVHYNSLTGE GNFNWRFLFP FQYLPAEKQM VITKRENIFS
1681LEKMECKTPA VLVLQVWDFE RLSSDDFLGT LEMNLNSFPR AAKSAKACDL AKFENASEET
1741KISIFQQKRV RGWWPFSKSK ELTGKVEAEF HLVTAEEAEK NPVGKARKEP EPLAKPNRPD
1801TSFSWFMSPF KCLYYLIWKN YKKYIIIAFI LIILIIFLVL FIYTLPGAIS RRIVVGS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1857 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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