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FDXR Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FDXR Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FDXR antibodies →
$725.00each
Catalog Number: LS-G116853-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Ferredoxin Reductase(FDXR) (NM_004110), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Adrenodoxin reductase | ADXR | FDXR | Ferredoxin--NADP(+) reductase | Ferredoxin reductase
Origin Species
Human
NCBI GenBank
NM_004110
SwissProt
P22570

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.48 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASRCWRWWG WSAWPRTRLP PAGSTPSFCH HFSTQEKTPQ ICVVGSGPAG FYTAQHLLKH
61PQAHVDIYEK QPVPFGLVRF GVAPDHPEVK NVINTFTQTA HSGRCAFWGN VEVGRDVTVP
121ELREAYHAVV LSYGAEDHRA LEIPGEELPG VCSARAFVGW YNGLPENQEL EPDLSCDTAV
181ILGQGNVALD VARILLTPPE HLEALLLCQR TDITKAALGV LRQSRVKTVW LVGRRGPLQV
241AFTIKELREM IQLPGARPIL DPVDFLGLQD KIKEVPRPRK RLTELLLRTA TEKPGPAEAA
301RQASASRAWG LRFFRSPQQV LPSPDGRRAA GVRLAVTRLE GVDEATRAVP TGDMEDLPCG
361LVLSSIGYKS RPVDPSVPFD SKLGVIPNVE GRVMDVPGLY CSGWVKRGPT GVIATTMTDS
421FLTGQMLLQD LKAGLLPSGP RPGYAAIQAL LSSRGVRPVS FSDWEKLDAE EVARGQGTGK
481PREKLVDPQE MLRLLGH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

497 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.