Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FCRLB / FREB-2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99277-100
Catalog Number: LS-G99277
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FCRLB (NM_001002901), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Fc receptor like 2 | Fc receptor-related protein Y | FCRLM2 | Fc receptor-like B | FCRLY | FCRLB | FREB-2 | FREB2 | RP11-474I16.6 | Fc receptor-like protein 2 | FcRY
- SwissProt
- Q6BAA4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.14 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRFWPVHQCA VYSGLTHGDF QVSSPIDVNG SLFWHQNQRD LPKCRNNAAP LTQMGGRRVR61WEVYISRARL VNRQNFVIRL TIGRREFMWP LTALLLLVPS SGQAATLEKP ILSLHPPWTT121IFKGERVTLK CDGYHPLLLE LQPISTLWYL GHLLLPSHKK SIEVQTPGVY RCQTRGAPVS181DPIHLSVSND WLILQVPYAP VFEGEPLVLR CRGWYDKVVY KLHYYHDGQA VRYFHSSANY241TVLQARASDS GRYQCSGTMR IPVESAPMFS AKVAVTVQEL FRAPVLRVMG PREARGAALG301GVVLRCDTRL HPQKRDTPLQ FAFYKYSRAV RRFDWGAEYT VPEPEVEELE SYWCEAATAT361RSVRKRSPWL QLPGPGSPLD PASTTAPAPW AAALAPGNRP LSFRKPPVSR SVPLVTSVRN421TTSTGLQFPA SGAPTAGPPA CAPPTPLEQS AGALKPDVDL LLREMQLLKG LLSRVVLELK481EPQALRELRG TPETPTSHFA VSPGTPETTP VESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
513 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.