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FCHSD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FCHSD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108214-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FCHSD1 (NM_033449), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FCH and double SH3 domains 1 | FCHSD1 | NWK2 | Nervous wreck homolog 2
Origin Species
Human
NCBI GenBank
NM_033449
SwissProt
Q86WN1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQPPPRKVKP AQEVKLRFLE QLSILQTWQQ READLLEDIR SYSKQRAAIE REYGQALQKL
61AGPFLKREGH RSGEMDSRGR TVFGAWRCLL DATVAGGQTR LQASDRYRDL AGGTGRSAKE
121QVLRKGTENL QRAQAEVLQS VRELSRSRKL YGQRERVWAL AQEKAADVQA RLNRSDHGIF
181HSRTSLQKLS TKLSAQSAQY SQQLQAARNE YLLNLVATNA HLDHYYQEEL PALLKALVSE
241LSEHLRDPLT SLSHTELEAA EVILEHAHRG EQTTSQVSWE QDLKLFLQEP GVFSPTPPQQ
301FQPAGTDQVC VLEWGAEGVA GKSGLEKEVQ RLTSRAARDY KIQNHGHRVL QRLEQRRQQA
361SEREAPSIEQ RLQEVRESIR RAQVSQVKGA ARLALLQGAG LDVERWLKPA MTQAQDEVEQ
421ERRLSEARLS QRDLSPTAED AELSDFEECE ETGELFEEPA PQALATRALP CPAHVVFRYQ
481AGREDELTIT EGEWLEVIEE GDADEWVKAR NQHGEVGFVP ERYLNFPDLS LPESSQDSDN
541PCGAEPTAFL AQALYSYTGQ SAEELSFPEG ALIRLLPRAQ DGVDDGFWRG EFGGRVGVFP
601SLLVEELLGP PGPPELSDPE QMLPSPSPPS FSPPAPTSVL DGPPAPVLPG DKALDFPGFL
661DMMAPRLRPM RPPPPPPAKA PDPGHPDPLT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

690 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.