LSBio
FBXW8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FBXW8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114615-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXW8 (NM_012174), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
F-box only protein 29 | FBXW6 | FBXW8 | FBW8 | FBW6 | FBX29 | FBXO29
Origin Species
Human
NCBI GenBank
NM_153348
SwissProt
Q8N3Y1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDDYSLDEFR RRWQEELAQA QAPKKRRRPE AAERRARRPE NEMNDVPFFD IQLPYELAIN
61IFQYLDRKEL GRCAQVSKTW KVIAEDEVLW YRLCQQEGHL PDSSISDYSC WKLIFQECRA
121KEHMLRTNWK NRKGAVSELE HVPDTVLCDV HSHDGVVIAG YTSGDVRVWD TRTWDYVAPF
181LESEDEEDEP GMQPNVSFVR INSSLAVAAY EDGFLNIWDL RTGKYPVHRF EHDARIQALA
241LSQDDATVAT ASAFDVVMLS PNEEGYWQIA AEFEVPKLVQ YLEIVPETRR YPVAVAAAGD
301LMYLLKAEDS ARTLLYAHGP PVTCLDVSAN QVAFGVQGLG WVYEGSKILV YSLEAGRRLL
361KLGNVLRDFT CVNLSDSPPN LMVSGNMDGR VRIHDLRSGN IALSLSAHQL RVSAVQMDDW
421KIVSGGEEGL VSVWDYRMNQ KLWEVYSGHP VQHISFSSHS LITANVPYQT VMRNADLDSF
481TTHRRHRGLI RAYEFAVDQL AFQSPLPVCR SSCDAMATHY YDLALAFPYN HV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

532 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.